All analyzes were performed in triplicate, unless for the food application test, and means and standard deviations were calculated, and when F values were significant (p 0.05) in the protein content of extracts obtained with the solid:liquid proportions of 1:12, 1:14, and 1:16, and the results were higher than the values obtained at lower dilution level (1:6, 1:8 and 1:10 ratios) (Figure 1C), so 1:12 was selected considering less water use
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Creating multiple lines isn't necessary, but it helps with your accuracy
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