Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Calcium Size:500g Glucose 20 Potato extract 4

Glucose 20 Potato extract 4 Agar 17 Suspend 41 gram powdered medium in 1 litre distilled water

92 g/L Sodium Hydroxide 4

pharmaceuticals

15 gram in 1 litre distilled water for 1X supplements

LoFlo vs VLoFlo: Triplicate biological repeats (cultures) Cell counts made to 48 h Note: by 26 h cells round and shrink* in both LoFlo and VLoFlo By 47 h cell rounding and shrinkage pronounced and assay stopped * Noted previously in Dicty community that LoFlo is not ideal for long-term culturing

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Calcium Size:500g Glucose 20 Potato extract 4Calcium chloride, CaCl2 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of calcium or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium Nitrate. Suspend 1. 8 gram powdered medium in 1 litre distilled water Store dry at room