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- Dephosphorylation of cloned vector DNA to prevent re-circularization during ligation
1 unit is the amount of enzyme required to bind 50% of 1 µg BstEII enzyme to the 12-base pair sticky end of λ DNA for 15 minutes at 45 ° C at a total reaction volume of 50 µl
purification of the fragmented double-stranded DNA can be performed within about 2 hours
or small amounts of purified genomic DNA)
allowing the synthesis of cDNA up to 20 kb in length
Custom PCR Master Mix With Tailored Label low rox - Dephosphorylation of cloned vectorYour protocol, your labelthis master mix is custom formulated to align with specialized workflows and arrives clearly marked for immediate identification. Personalized reagent composition meets unique experimental requirements Professional, laboratory grade labeling includes your chosen identifiers Meticulously QC tested for batch to batch reproducibility and traceability
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