Pre Sporulation Medium Size:250g minus Cysteine

minus Cysteine

1 Bottle contains 20 ml G-418 Solution 50 mg/ml

4% stock solution in ethanol) of 9 ml per litre medium has to be added prior to autoclavation

SD Broth / 2% Glucose w/o Phosphate (KH2PO4) is based upon Yeast Nitrogen base without Amino acids and including glucose

Yeast extract 10 Peptone 20 Galactose 20 Agar 20 Suspend 70 gram powdered medium in 1 litre distilled water

Pre Sporulation Medium Size:250g minus CysteinePre Sporulation Medium is used for strains that do not sporulate well when grown on sporulation medium directly. Cells are grown 1 2 days on pre sporalation medium before transferring them to sporulation medium. Formula g l Yeast extract 10 Glucose 100 Potassium Acetate 20 Agar 20 Suspend 150 gram powdered medium in 1 litre distilled water Store dry at room temperature.