Leptosphaeria maculans Probe Realtime PCR Kit (without internal control) Nucleic Acids & Derivatives and data analysis settings for

and data analysis settings for ABI7500 (can be adapted to other instruments)

Parameter Details Nominal concentration (format) 10 CFU per tube (NNE002) and 100 CFU per tube (NNE001) Tube count per kit 3 tubes of mycobacterium standard + 3 tubes DNA Dilution Buffer per kit DNA Dilution Buffer (DDB) 1

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as this may reduce PCR efficiency

Numerous pseudogenes have been reported for this gene

Leptosphaeria maculans Probe Realtime PCR Kit (without internal control) Nucleic Acids & Derivatives and data analysis settings forDescription Leptosphaeria maculans Probe Realtime PCR Kit is a PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe based real time PCR. Purified DNA is directly amplified using sequence specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real time monitoring of amplification. The resulting signals may be used for comparative